Данные расходы планируются в первый

Данные расходы планируются в первый год выполнения проекта.
Для измерения экспресии генов воспаления и апоптоза необходимо приобрести TRIzol® (Invitrogen, США) и ImProm-II™ Reverse Transcription System kit (Promega, USA), рекомбинантный RNasin(R) RNase Inhibitor, обратную транскриптазу ImProm-II(TM) Reverse Transcriptase, а также заказать синтез праймеров. Данные расходы планируются в первый и третий год выполнения проекта.
Для измерения степени гетероплазмии мтДНК методом пиросеквенирования необходимо приобрести наборы для выделения ДНК Aquapure Genomic Tissue Kit (Bio-Rad), Taq полимеразу, наборы PyroMark Gold Q96 Reagents (Qiagen), стрептавидин-сефарозу HP (Qiagen), фильтры для устройства вакуумной пробоподготовки PyroMark Q24 (Qiagen), картриджи для прибора PyroMark Q96 (Qiagen), микропланшеты PyroMark Q96 (Qiagen), а также заказать синтез прямых, обратных и сиквенс-праймеров. Данные расходы планируются в первый и второй год выполнения проекта.
Для изучения вариабельности мтДНК методом полногеномного секвенирования на платформе Roche 464 необходимо приобрести наборы для обогащения мтДНК REPLI-g Mitochondrial Kit (Qiagen), наборы для быстрого приготовления библиотек GS Rapid Library Prep Kit, наборы для эмПЦР-лигированных библиотек GS Junior Titanium emPCR Kit (Lib-L), наборы для секвенирования GS Junior Titanium Sequencing Kit (Roche). Данные расходы планируются в первый и второй год выполнения проекта.

Результаты (английский) 1:

These costs are planned in the first year of the project.
for the measurement of the èkspresii genes of apoptosis and inflammation should purchase TRIzol ® (Invitrogen, United States) and ImProm-II ™ Reverse Transcribed System kit (°, USA), recombinant RNasin (R), An RNase back to transkriptazu ImProm-II (TM) Reverse Transcriptase, and the synthesis of primers. These costs are in the first and third year of the project.
To measure the mtDNA method geteroplazmii pyrosequencing to purchase kits for DNA Genomic Aquapure selections Tissue Kit (Bio-Rad), Taq polymerase, PyroMark Gold Q96 Reagents kits (Qiagen) and streptavidin-sefarozu HP (Qiagen) filters for vacuum sample preparation device PyroMark waste waters per day-(Qiagen), cartridges for appliance PyroMark Q96 (Qiagen), mikroplanšety PyroMark Q96 (Qiagen), as well as a synthesis of direct, inverse and sequence-primers. These costs are in first and second year of the project.
To study the variability of mtDNA sequencing on the polnogenomnogo method of the Roche 464 to purchase kits for mtDNA REPLI-g Mitochondrial Kit (Qiagen) quick-cooking kits Rapid Library GS library Prep Kit, èmPCR-ligirovannyh libraries GS Junior Titanium emPCR Kit (Lib-L), sequencing kits GS Junior Titanium Sequencing Kit (Roche). These costs are in first and second year of the project.

Результаты (английский) 2:

These expenses are scheduled in the first year of the project.
for measuring gene expression of inflammation and apoptosis is necessary to acquire TRIzol ® (Invitrogen, USA) and ImProm-II ™ Reverse Transcription System kit (Promega, USA), recombinant RNasin (R) RNase Inhibitor, reverse transcriptase ImProm-II (TM) Reverse Transcriptase, and order synthesis primers. These expenses are planned in the first and third year of the project.
To measure the degree of mtDNA heteroplasmy pyrosequencing must purchase kits for DNA extraction Aquapure Genomic Tissue Kit (Bio-Rad), Taq polymerase kits PyroMark Gold Q96 Reagents (Qiagen), streptavidin-Sepharose HP (Qiagen), filters for the vacuum sample preparation PyroMark Q24 (Qiagen), cartridges for the device PyroMark Q96 (Qiagen), microplates PyroMark Q96 (Qiagen), and order direct synthesis, inverse and sequencing primers. These expenses are planned for the first and second year of the project.
To study the variability of mtDNA genome-sequencing method on Roche 464 platform must purchase kits to enrich mtDNA REPLI-g Mitochondrial Kit (Qiagen), sets noodles libraries GS Rapid Library Prep Kit, kits emPTsR-ligated libraries GS Junior Titanium emPCR Kit (Lib-L), kits for sequencing GS Junior Titanium Sequencing Kit (Roche). These expenses are planned for the first and second year of the project.

Результаты (английский) 3:

Data costs are planned in the first year the project.
For measuring экспресии genes inflammation and other purpose must be to purchase TRIzоl® (Invitrоgen, USA) and ImPrоm-II™ Reverse transcription system kit (Prоmegа, USA), Rinsulin nph RNаsin(R) RNаse NumberFeedback транскриптазу ImPrоm-II(TM) Reverse Trаnsсriptаse, as well as ordered synthesis primaries. Data costs are planned in the first and the third year the project.
To measure the degree to which гетероплазмии мтДНК пиросеквенирования method you will need to purchase kits for DNA Aquаpure Genоmiс tissue Kit ( Biо-Rаd ), Tаq полимеразу, sets PyrоMаrk Gold Q96 Reаgents (Qiаgen), стрептавидин-сефарозу HP (Qiаgen),Filters for the device vacuum Chromatech SDB PyrоMаrk Q24 (Qiаgen), cartridges for the appliance PyrоMаrk Q96 (Qiаgen), микропланшеты PyrоMаrk Q96 (Qiаgen), as well as ordered synthesis direct, inverse and сиквенс-primaries.Data costs are planned in the first and the second year the project.
To study the variability мтДНК method полногеномного neuraminidase inhibitors on the platform for fancy boxes 464 it is necessary to purchase kits for enrichment мтДНК REPLI-g Mitосhоndriаl Kit (Qiаgen),Kits for fast cooking libraries GS Rapid Library Prep Kit, kits for эмПЦР-лигированных libraries GS Junior titanium emPCR Kit (Lib-L), sets for rnai)GS Junior titanium sequencing Kit (fancy boxes).Data costs are planned in the first and second year the execution of the project.