Для выделения моноцитов и работы с

Для выделения моноцитов и работы с первичной культурой клеток необходимо приобрести среду LSM для разделения лимфоцитов (MP Biomedicals), Ficoll-Paque (GE Healthcare Life Sciences), аффинные парамагнитные микрочастицы CD14 MicroBeads (Miltenyl Biotec), разделительные колонки LS Separation Columns (Miltenyl Biotec), среду X-Vivo 10 (Lonza), компоненты для питательных сред. Данные расходы планируются во второй и третий год выполнения проекта.
Для оценки функциональной активности клеток необходимо приобрести наборы ключевых реагентов Human TNF-alpha/TNFSF1A (R&D Systems), набор ключевых реагентов для определения Human CCL18/PARC DuoSet (R&D Systems), cубстрат Substrate Reagent Pack (R&D Systems), рекомбинатный человеческий ИЛ-4 (ProSpec), рекомбинатный человеческий интерферон-гамма (ProSpec), [3H]-тимидин, [14С]-лейцин, [3H]-пролин, наборы для определения холестерина (Analyticon). Данные расходы планируются в третий год выполнения проекта.
Необходимы закупки расходных материалов (наконечники для дозаторов, лабораторный пластик, планшеты для ИФА, планшеты для ПЦР, пробирки, культуральные чашки, стрипы для ПЦР с крышками). Данные расходы планируются в течение всего времени выполнения проекта.

Результаты (английский) 1:

For monocytes and work with primary culture cells need to purchase separate LSM medium lymphocytes (MP Biomedicals), Ficoll-Paque (GE Healthcare Life Sciences), affine ParaMagnetic microparticles CD14 MicroBeads (Miltenyl Biotec) separating columns of LS Separation Columns (Miltenyl Biotec), the X 10-Vivo environment (Lonza), components of culture media. These costs are planned in the second and third year of the project.
To assess the functional activity of cells need to purchase sets of key reagents of Human TNF-alpha/TNFSF1A (R & D Systems), a set of key reagents for determining Human CCL18/PARC DuoSet (R & D Systems), cubstrat Substrate Reagent Pack (R & D Systems), recombinant human IL-4 (ProSpec), recombinant human interferon-gamma (ProSpec), [3 h]-thymidine, [14 c] Leucine, [3 h] PROLINE, kits for determination of cholesterol (Analyticon). These costs are in the third year of the project.
Requires the purchase of consumables (ferrules for batchers, laboratory plastic, tablets for IFA, PCR tablets, vials, culture Cup, strips for PCR with covers). These costs are planned throughout the lifetime of the project.

Результаты (английский) 2:

To isolate monocytes and work with primary cell culture medium must purchase LSM lymphocyte separation (MP Biomedicals), Ficoll-Paque (GE Healthcare Life Sciences), affine paramagnetic microparticles CD14 MicroBeads (Miltenyl Biotec), separation columns LS Separation Columns (Miltenyl Biotec) , medium X-Vivo 10 (Lonza), components of culture media. These expenses are planned in the second and third year of the project.
To assess the functional activity of cells need to purchase sets of key reagents Human TNF-alpha/TNFSF1A (R & D Systems), a set of key reagents for the determination of Human CCL18/PARC DuoSet (R & D Systems), cubstrat Substrate Reagent Pack (R & D Systems), recombinant human IL-4 (ProSpec), recombinant human interferon-gamma (ProSpec), [3H]-thymidine, [14C] leucine, [3H]-proline, kits for the determination of cholesterol (Analyticon). These expenses are planned for the third year of the project.
required purchase of consumables (pipette tips for, laboratory plastic plates for ELISA, PCR plates, test tubes, culture plates, strips for PCR with lids). These expenses are planned for the entire time of the project.

Результаты (английский) 3:

To highlight моноцитов and work with primary culture cells need to buy environment LSM to separate esr (MP Biоmediсаls), Fiсоll-Pаque (GE Healthcare Life Sciences), affine a paramagnetic conceptually CD14 MiсrоBeаds (Miltenyl 'RIA Novosti' newswire).Separator column LS separation Columns (Miltenyl 'RIA Novosti' newswire), Wednesday X-vivo 10 (Lоnzа), components for nutrient environments. Data costs are planned in the second and the third year the project.
To assess functional activity cells need to buy sets key reagents human TNF-alpha/TNFSF1A (R&D Systems), a set of key reagents for determining human CCL18/PARC DuоSet (R&D Systems), substrates dorsal side Reаgent Pack (R&D Systems),Рекомбинатныи human IL-4 (PrоSpeс), рекомбинатныи human interferon-gamma (PrоSpeс), (3H) -тимидин, (14C) -леицин, (3H) -Proline, kits to determine the cholesterol (Anаlytiсоn).Data costs are planned in the third year of implementation of draft.
needed supplies procurement (tips for meters, laboratory plastic, tablets for ELISAS, tablets for PCR, surrogacy,Including Médecins cup, ea-d band is positive for PCR with the covers). Data costs are planned for the entire duration of the project.