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английский) 2:
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those analysis of the p. h'rmala alkaloid y performrad would rp-plk sylw diod arre detector uzing weters lk 2695 coupe to weather 996 diode arre detector (dad). 100 × 4.6 mm and.., 5 zm, sterra ms-18 columun (agilent technology) y sed for analysis. chromatographic conditioning ware: w'ter supreteted whit triethyl amin (f 8.6) () and aketonitril (b). those gradient y programd from 0% b (100% ) then 20% b in 4 min, ten 30% b at 9 mins, 50% b at 14 mins, 50% b at 14 mins Ande finales then 60% b at 16 min separat y folloued y 2 min wassing prosedura whit 100% b and re-equilibration period of 4 min.those flow rate y 1 ml/min, those colum The temperature at 25 degrees and those injections of will at 20 il. under thesis condition those elution y would warrant: peganin, h'rmol and h'rmaline. abstract detection y curryd out at 320 nm.
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