Выделение и культура in vitroСтволо

Выделение и культура in vitro
Стволовые клетки были выделены при анализе тератокарциномы. В 1964 году исследователи показали, что клетки тератокарциномы остаются недифференцированными в культуре клеток. Такие стволовые клетки называют эмбриональными клетками карциномы. Исследователи показали, что первичные эмбриональные зародышевыеклетки могут размножаться в культуре и могут образовывать разные типы клеток.
Эмбриональные стволовые клетки выделили из мышиных эмбрионов в 1981 году Мартин Эванс и Мэтью Кауфман, а также независимо от них Гэйл Мартин. Прорыв в исследовании эмбриональных стволовых клеток человека произошел в ноябре 1998 года в группе Джеймса Томсона в Университете Висконсина. Ученые выделили такие клетки из бластоцисты человека.
Загрязнения веществами, которые используют для поддержания клеточных культур
Сетевая версия журнала Nature Medicine опубликовало в январе 2005 года материал, согласно которому стволовые клетки человека, которые доступны для исследований, финансируемых федеральными грантами, загрязнены молекулами сред для культивирования клеток животного происхождения. Для поддержания плюрипотентности активно делящихся клеток часто используют клетки животного происхождения (обычно это клетки мышей). Выяснилось, что это порождает ряд проблем; в частности, оказалось, что сиаловая кислота животного происхождения сужает возможности применения эмбриональных стволовых клеток для терапевтических целей.
В работе, опубликованной в журнале Lancet Medical Journal 7 марта 2005 года, детально описана методика культивирования новой линии стволовых клеток в среде, полностью свободной от клеток и сыворотки животного происхождения. После более чем шестимесячного периода культивирования в недифференцированном состоянии эти клетки могли дифференцироваться в клетки всех трех зародышевых листков как в составе тератом, так и в культуре.

Результаты (английский) 1:

Selection and culture in vitroStem cells were allocated when parsing teratokarcinomy. In 1964 year researchers have shown that teratokarcinomy cells remain undifferentiated in culture cells. These stem cells are called embryonic carcinoma cells. Researchers have shown that primary embryonic zarodyshevyekletki can multiply in culture and can form different types of cells.Embryonic stem cells from mouse embryos singled out the 1981 year Martin Evans and Matthew Kaufman, as well as independently Gail Martin. Breakthrough in the study of embryonic stem cells took place in November 1998 the year of James Thomson's group at the University of Wisconsin. Scientists have identified such cells from human blastocysts.Contamination with substances which are used for maintenance of cell culturesThe network version of the journal Nature Medicine published in January 2005 of the year material, according to which human stem cells available for research funded by federal grants, polluted molecules Wednesday for cultivation of cells of animal origin. To maintain the pluripotency of actively dividing cells often use animal cells (usually cells mice). It turned out that this raises a number of problems; in particular, it turned out that the sialic acid of animal origin constrained use of embryonic stem cells for therapeutic purposes.In the work, published in the journal Lancet Medical Journal March 7, 2005 year, detailed methodology cultivation of new lines of stem cells in Wednesday, completely free from cells and animal serum. After more than six months in an undifferentiated State of these cells can differentiate into cells of all three embryonic leaves both in teratom and in culture.

Результаты (английский) 2:

Isolation and in vitro culture of
stem cells have been isolated in the analysis teratocarcinoma. In 1964, researchers showed that cells remain undifferentiated teratocarcinoma cell culture. Such cells are called stem cells, embryonic carcinoma. Researchers have shown that primary embryonic zarodyshevyekletki in culture can proliferate and can form various types of cells.
Embryonic stem cells are isolated from mouse embryos in 1981 by Martin Evans and Matthew Kaufman and independently by Gail Martin. A breakthrough in the research of human embryonic stem cells occurred in November 1998 in the group of James Thomson at the University of Wisconsin. Scientists have identified such cells from human blastocysts.
Pollution substances, which are used for maintenance of cell cultures
Network version of the journal Nature Medicine published in January 2005. The material, according to which human stem cells, which are available for research funded by federal grants, contaminated molecules media for culturing animal cells. To maintain pluripotency of actively dividing cells often use animal cells (usually a mouse cells). It turned out that this gives rise to a number of problems; In particular, it was found that the sialic acid animal narrows the possibilities of application of embryonic stem cells for therapeutic purposes.
In a paper published in the journal Lancet Medical Journal March 7, 2005, describes a method of culturing detail new lines of stem cells in a medium completely free of animal cells and serum. After more than six months of culture in an undifferentiated state these cells can differentiate into cells of all three germ layers both in structure teratomas, and in culture.

Результаты (английский) 3:

separation and culture in vitrostem cells have been identified in the analysis of тератокарциномы. in 1964, researchers have shown that cells тератокарциномы remain недифференцированными in culture cells. such stem cells called embryonic cells of carcinoma. researchers have shown that primary embryonic зародышевыеклетки can propagate in culture and may form different cell types.embryonic stem cells isolated from murine embryos in 1981, martin evans, and matthew kaufman, and independent of the gail martin. a breakthrough in the study of human embryonic stem cells took place in november 1998 in the band james thomson at the university of wisconsin. scientists have identified such cells from бластоцисты rights.pollution substances, which is used for the maintenance of cell culturesthe network version of the journal nature medicine published in january 2005, material, according to which human stem cells, which are available for research funded by federal grants, contaminated molecules for cultivation of animal cells. in order to maintain the плюрипотентности actively fertile cells often use cells of animal origin (usually it is the cells of mice). it turns out that this raises a number of issues; in particular, it turns out that the sialic acid of animal origin reduces the possibility of the use of embryonic stem cells for therapeutic purposes.the work, published in the lancet medical journal on march 7, 2005, in detail, describes the method of cultivation of new lines of stem cells in an environment completely free of cells and serum of animal origin. after more than six months of cultivation in an undifferentiated state of these cells could differentiate into cells of all three stem pieces as the тератом, and culture.